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KMID : 1011420110160010091
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Journal of Korean Ophthalmic Optics Society
2011 Volume.16 No. 1 p.91 ~ p.95
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Selective Accumulation of Rhodamine B in M?ler cells in Rabbit Retina
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Kwon Oh-Ju
Lee Eun-Shil
Jeon Chang-Jin
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Abstract
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Purpose: In this study, we investigated the dye to staining for selective accumulation in rabbit retina.
Methods: Rhodamine B was injected into the vitreous body in rabbit. After 24 h, the isolated retina was checked condition of cell staining on the microscope. We used conventional immunocytochemical techniques for recognizing cell type.
Results: Well-labeled nuclei were seen in the middle of the inner nuclear layer of the rabbit retina. The number and distrbution of the accumulating cells were similar to those of the m?ler glia. To identify m?ler cell, we used antibody directed against vimentin. Rhodamine B-immunoreactive nuclei also were labeled with anti-vimentin antibody. We found that Rhodamine B was accumulated selectively in retinal m?ler cell.
Conclusions: Specific accumulation in rabbit retinal m?ler cell occurred when Rhodamine B was applied to living retina.
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KEYWORD
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Cell staining, M?ler cell, Rabbit retinal cell, Rhodamine B
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